Protein Biochemistry/Molecular Biology
The Central Dogma of Molecular Biology proposed by Francis Crick in 1958, explains how genetic information is transferred from DNA to RNA to proteins. DNA functions as a storage medium for the information necessary to synthesize proteins, and RNA is responsible for the translation of this information into protein molecules, as part of the ribosome.
Gene Cloning
We have a team of experienced scientists who can carry out the following spectrum of cloning techniques
- Preparation of genomic DNA
- Cloning of full length cDNA: Amplification of specific cDNA by RT-PCR and cloning into a vector of your choice
- Cloning of PCR products: Amplification of specific DNA fragments by PCR and cloning into a standard vector or into a vector of your choice
- Subcloning of vector inserts: Subcloning of DNA fragments into target vectors, e.g. for in vivo protein expression, or for construction of hybrid genes
- Cloning of synthetic genes: De novo synthesis of DNA fragments or complete genes and cloning into a standard vector or a vector of your choice, e.g. expression vectors
Please contact us to discuss on your cloning needs
Protein Expressions
Mammalian cell expression systems, bacterial cell expression systems, yeast expression systems, insect cell expression systems and plant cell expression systems are the five state of the art technologies that the company will strive to build its expertise. Indus Gene Expressions seeks partnership with academic and industrial researchers to develop contract research projects, produce challenging proteins failed elsewhere.
Protein Purification
Purity of the protein is an important factor in various R&D and industrial applications. Indusgene expressions have expertise scientists to carry out protein purification using the following purification and analytical techniques
- Ion-exchange chromatography
- Affinity chromatography
- Size-exclusion chromatography
- AKTA-FPLC, RP-HPLC
- Other associated techniques
